| Date | Home | Away | Result |
|---|---|---|---|
| 9/26/25 1:30 PM | US Catanzaro 1929 | Juve Stabia | 2-2 |
| 9/27/25 8:00 AM | US Avellino | Virtus Entella | 2-0 |
| 9/27/25 8:00 AM | Mantova 1911 | Frosinone Calcio | 1-5 |
| 9/27/25 8:00 AM | FC Cesena | Palermo FC | 1-1 |
| 9/27/25 8:00 AM | FC Südtirol | AC Reggiana 1919 | 3-1 |
| 9/27/25 8:00 AM | FC Venice | Spezia Calcio | 2-0 |
| 9/27/25 10:15 AM | AC Monza | Padova Calcio | 0-1 |
| 9/27/25 12:30 PM | SSC Bari | Sampdoria Genua | 1-1 |
| 9/28/25 10:15 AM | Modena FC | Pescara Calcio | 2-1 |
| 9/28/25 12:30 PM | FC Empoli | Carrarese Calcio 1908 | 2-2 |